Major Goals and Objectives

• Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
• Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
• Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
• Use the ES cell cultures to generate zebra fish aromatase knockout
  lines of fish

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