• The goal of this project was to use a direct method for investigating the speciation of metals in wetlands that is based on the use of Synchrotron Radiation

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• To determine the extent of dietary overlap between bighead carp and native filter-feeding fishes in the Mississippi and Illinois river systems
• To use data from the Long Term Resource Monitoring Program to examine whether specific environmental factors correlate with successful reproduction of bighead carp in the Upper Mississippi River System
• To test the effectiveness of an electric barrier in restricting the spread of bighead carp

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• Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
• Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
• Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
• Use the ES cell cultures to generate zebra fish aromatase knockout
  lines of fish

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• Evaluate the effectiveness of electric barriers in restricting the movement of bighead and/or silver carp
• Evaluate the effectiveness of acoustic/bubble barriers in restricting the movement of bighead and/or silver carp
• Evaluate the effectiveness of combined barrier types (i.e. electric / acoustic / bubble) in restricting the movements of bighead and/or silver carp

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• Monitor environmental factors and larval zebra mussel abundances in the Hudson River and contrast conditions in embayments and in the main river channel
• Conduct dye studies near embayments to determine their ability to retain zebra mussel larvae
• Use data from the Hudson River and our previous data from the Illinois and Hudson Rivers to determine whether side embayments can act as sources or sinks for zebra mussel larvae

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