• Determine the concentrations and speciation of PFAS in important Lake Michigan sportfish
• Evaluate dietary routes for PFAS exposure from prey to predator fish using δ15N and δ13C stable isotopes along with PFAS speciation
• Assess the relationship between total fluorine and PFAS concentrations to determine the full extent of the PFAS problem
• Compare observed PFAS concentrations in fish from Illinois-Indiana waters to other areas such as inland Michigan waterways where PFAS are known to be a problem

• Enhance the NAPRA online web-based decision support tool and update the soil database using SSURGO soils data suitable for field and county level evaluation
• Assess the level of phosphorus reaching water with existing cropping management systems and assess the change in phosphorus level due to increases in biofeedstock production and intensive livestock production

This project aims to investigate the potential role of marine plastic debris as a vector for Perfluorinated Compounds (PFAS). Specific research questions are:

• To what extent do three common plastic types found in the environment act as a sink for select PFASs in Muskegon Lake?
• How important is the residence time of the materials present in these water bodies concerning the extent of PFAS accumulation?

The objectives of this study are to:

• Study the sources and contamination levels of enteric bacteria and viruses in Burns Ditch, one of the most polluted streams that flow into Lake Michigan in Northwest Indiana.
• Determine the impact of the pollution of Burns Ditch on the microbial quality of lake front water off West Beach, located three kilometers west of the water outfall.
• Use a molecular approach to detect viruses in stream and lake waters.
• Use a computer-based image analysis system to determine the quantity of viruses in water samples.

• Examine drinking water disinfection byproducts for ability to kill mammalian cells and damage mammalian DNA
• Compare results to published databases that include carcinogens

• Grow freshwater actinomycete bacteria from sediment collected in Lake Michigan and other Great Lakes

• To test the hypothesis that human and animal E. coli strains are distinguishable
• To screen and select appropriate primers
• To build a small RAPD (random amplied polymorphic DNA) fingerprint database for E. coli from human and nonhuman sources
• To identify landmark DNA patterns
• To determine the sensitivity and applicability of this technology