This project aims to investigate the potential role of marine plastic debris as a vector for Perfluorinated Compounds (PFAS). Specific research questions are:

• To what extent do three common plastic types found in the environment act as a sink for select PFASs in Muskegon Lake?
• How important is the residence time of the materials present in these water bodies concerning the extent of PFAS accumulation?

The objectives of this study are to:

• Study the sources and contamination levels of enteric bacteria and viruses in Burns Ditch, one of the most polluted streams that flow into Lake Michigan in Northwest Indiana.
• Determine the impact of the pollution of Burns Ditch on the microbial quality of lake front water off West Beach, located three kilometers west of the water outfall.
• Use a molecular approach to detect viruses in stream and lake waters.
• Use a computer-based image analysis system to determine the quantity of viruses in water samples.

• To test the hypothesis that human and animal E. coli strains are distinguishable
• To screen and select appropriate primers
• To build a small RAPD (random amplied polymorphic DNA) fingerprint database for E. coli from human and nonhuman sources
• To identify landmark DNA patterns
• To determine the sensitivity and applicability of this technology