- Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
- Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
- Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
- Use the ES cell cultures to generate zebra fish aromatase knockout
lines of fish
Results
Aquatic nuisance species: an evaluation of barriers for preventing the spread of Bighead and Silver carp to the Great Lakes
- Evaluate the effectiveness of electric barriers in restricting the movement of bighead and/or silver carp
- Evaluate the effectiveness of acoustic/bubble barriers in restricting the movement of bighead and/or silver carp
- Evaluate the effectiveness of combined barrier types (i.e. electric / acoustic / bubble) in restricting the movements of bighead and/or silver carp
Metapopulation dynamics and control of the zebra mussel in freshwater and estuarine systems: the effects of hydrodynamics, larval supply, and embayments
- Monitor environmental factors and larval zebra mussel abundances in the Hudson River and contrast conditions in embayments and in the main river channel
- Conduct dye studies near embayments to determine their ability to retain zebra mussel larvae
- Use data from the Hudson River and our previous data from the Illinois and Hudson Rivers to determine whether side embayments can act as sources or sinks for zebra mussel larvae
Development of Water Markets for Northeastern Illinois
- Assess water supply management options for northeastern Illinois
DNA fingerprinting as a means for tracing the source of E. coli contamination
- To test the hypothesis that human and animal E. coli strains are distinguishable
- To screen and select appropriate primers
- To build a small RAPD (random amplied polymorphic DNA) fingerprint database for E. coli from human and nonhuman sources
- To identify landmark DNA patterns
- To determine the sensitivity and applicability of this technology
Consequences of Round Goby Invasion for Littoral Zone Communities: Effects on Sculpin and Benthic Invertebrates Project
- Examine diets of sympatric and allopatric populations of mottled sculpins and round gobies
- Determine the relationship between mottled sculpin and round goby feeding on benthic invertebrate
communities - Assess the role of round gobies as trophic replacements for mottled sculpins
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