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Targeted inactivation of the fish aromatase gene

Principal Investigator: Paul Collodi
Affiliation: Purdue University
Initiation Date: 2002
  • Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
  • Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
  • Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
  • Use the ES cell cultures to generate zebra fish aromatase knockout
    lines of fish

Aquatic nuisance species: an evaluation of barriers for preventing the spread of Bighead and Silver carp to the Great Lakes

Principal Investigator: Mark Pegg
Affiliation: Illinois Natural History Survey
Initiation Date: 2001
  • Evaluate the effectiveness of electric barriers in restricting the movement of bighead and/or silver carp
  • Evaluate the effectiveness of acoustic/bubble barriers in restricting the movement of bighead and/or silver carp
  • Evaluate the effectiveness of combined barrier types (i.e. electric / acoustic / bubble) in restricting the movements of bighead and/or silver carp

Metapopulation dynamics and control of the zebra mussel in freshwater and estuarine systems: the effects of hydrodynamics, larval supply, and embayments

Principal Investigator: Dianna Padilla
Affiliation: State University of New York
Initiation Date: 2001
  • Monitor environmental factors and larval zebra mussel abundances in the Hudson River and contrast conditions in embayments and in the main river channel
  • Conduct dye studies near embayments to determine their ability to retain zebra mussel larvae
  • Use data from the Hudson River and our previous data from the Illinois and Hudson Rivers to determine whether side embayments can act as sources or sinks for zebra mussel larvae

DNA fingerprinting as a means for tracing the source of E. coli contamination

Principal Investigator: Charles Tseng
Affiliation: Purdue University Calumet
Initiation Date: 2000
  • To test the hypothesis that human and animal E. coli strains are distinguishable
  • To screen and select appropriate primers
  • To build a small RAPD (random amplied polymorphic DNA) fingerprint database for E. coli from human and nonhuman sources
  • To identify landmark DNA patterns
  • To determine the sensitivity and applicability of this technology

Consequences of Round Goby Invasion for Littoral Zone Communities: Effects on Sculpin and Benthic Invertebrates Project

Principal Investigator: Martin Berg
Affiliation: Loyola University Chicago
Initiation Date: 2000
  • Examine diets of sympatric and allopatric populations of mottled sculpins and round gobies
  • Determine the relationship between mottled sculpin and round goby feeding on benthic invertebrate
    communities
  • Assess the role of round gobies as trophic replacements for mottled sculpins

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