- Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
- Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
- Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
- Use the ES cell cultures to generate zebra fish aromatase knockout
lines of fish
Results
Bighead carp in the upper midwest river: competition with native filter-feeding fishes and potential threats to the Great Lakes
- To determine the extent of dietary overlap between bighead carp and native filter-feeding fishes in the Mississippi and Illinois river systems
- To use data from the Long Term Resource Monitoring Program to examine whether specific environmental factors correlate with successful reproduction of bighead carp in the Upper Mississippi River System
- To test the effectiveness of an electric barrier in restricting the spread of bighead carp
Mercury methylation and demethylation in a contaminated urban watershed
Speciation and availability of metals in wetlands
- The goal of this project was to use a direct method for investigating the speciation of metals in wetlands that is based on the use of Synchrotron Radiation
Economic impact of ballast-mediated invasive species in the Great Lakes
- Determine the ecological changes caused by nonindigenous species that were introduced and established in the Great Lakes via ship ballast discharges
- Quantify the minimum net financial cost (based on market costs) imposed on
the Great Lakes region by shipping-mediated nonindigenous species - Forecast the potential range in North America for a subset of high risk species
- Assess how unquantified costs (for currently unstudied species, for market costs imposed outside the Great Lakes region, for non-market costs imposed within and outside the Great Lakes region) might change our evaluation of the net cost of the species and net value of shipping
- Develop policy recommendations and an outreach strategy to inform the public and policy-makers as decisions are made about the management of ballast and the St. Lawrence Seaway
Aquatic nuisance species – evaluating the ecological and economic value of the 100th Meridian Initiative
- In what parts of the Columbia and Colorado river and reservoir systems are zebra mussels likely to thrive if they are introduced from infested eastern waters?
- For those parts of the target river systems that meet the ecological requirements of zebra mussel, what a) environmental goods and services, and b) financial and commercial goods and services are at risk?
- What are the costs and how effective are different prevention strategies? For the 100th Meridian Initiative, what is the most cost-effective level of investment in prevention and control?
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