According to some of the latest research, a population of Asian carp as small as 20 in number could be enough to establish a damaging population of the invasive species in the Great Lakes. 
From The Atlantic Cities
“The report notes that this small population would have a greater than 50 percent chance of successfully spawning if they were to find a viable river access point to the lakes. Lake Michigan is the most likely target, and the Chicago Area Waterway System of rivers, sanitary canals and locks would be the most likely entry point. Already, the environmental DNA of carp has been detected as close as six miles from Lake Michigan. Some worry that the carp’s entrance into the Great Lakes is inevitable.”
Read more, including links to the full report and additional information, at the link above.
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