• Evaluate the potential use of ethanol yeast, a co-product of bio-ethanol production, as a fish meal replacement in the diets of sunshine bass (white bass Morone chrysops x striped bass M. saxatilis)

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• Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
• Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
• Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
• Use the ES cell cultures to generate zebra fish aromatase knockout
  lines of fish

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• Obtain the full-length perch growth hormone (GH) cDNA.
• Clone and express the yellow perch GH mRNA in a protein expression system.
• Assay the recombinant perch GH for growth promoting activity in juvenile perch.
• Produce antibodies to the recombinant perch GH.

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The objective of this project is to characterize perch effluent water quality from two different by similarly designed recirculating aquaculture systems.

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