- Establish methods for the culture of fish primordial germ cells (PGCs)
Results
Replacement of fish meal in hybrid striped bass diets with proteinaceous fermentation biomass
- Evaluate the potential use of ethanol yeast, a co-product of bio-ethanol production, as a fish meal replacement in the diets of sunshine bass (white bass Morone chrysops x striped bass M. saxatilis)
Targeted inactivation of the fish aromatase gene
- Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
- Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
- Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
- Use the ES cell cultures to generate zebra fish aromatase knockout
lines of fish
The development of molecular and biochemical tools to assess changes in yellow perch (Perca flavescens) growth hormone
- Obtain the full-length perch growth hormone (GH) cDNA.
- Clone and express the yellow perch GH mRNA in a protein expression system.
- Assay the recombinant perch GH for growth promoting activity in juvenile perch.
- Produce antibodies to the recombinant perch GH.
Yellow Perch Effluent Characterization
The objective of this project is to characterize perch effluent water quality from two different by similarly designed recirculating aquaculture systems.
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