• Develop and deliver an introductory fish health class for veterinarians and veterinary students, fisheries professionals and students, and other stakeholders in Illinois and Indiana

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• Initiate embryonic stem (ES) cell cultures derived from zebra fish embryos and maintain the cultures for multiple passages in vitro
• Construct targeting vectors that can be used to disrupt the zebra fish aromatase genes
• Introduce the targeting vectors into the ES cells and develop methods to isolate homologous recombinants
• Use the ES cell cultures to generate zebra fish aromatase knockout
  lines of fish

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To evaluate the effect of culture temperature on growth, survival, and body composition of largemouth bass.

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• Obtain the full-length perch growth hormone (GH) cDNA.
• Clone and express the yellow perch GH mRNA in a protein expression system.
• Assay the recombinant perch GH for growth promoting activity in juvenile perch.
• Produce antibodies to the recombinant perch GH.

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• Establish methods for the culture of fish primordial germ cells (PGCs)

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• Evaluate the polyculture of yellow perch in cages with freshwater prawns in ponds.
• Evaluate the effect of culture temperature on growth and survival of yellow perch.

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